Tailor made services based on your research project requirements
Recombinant Protein Expression & Purification
We proudly offer this service for investigators who don’t have gene or construct for their needed recombinant protein expression and purification. We’ll PCR-amplify gene or if needed synthesize the optimized gene, sub-clone it into an expression vector and express it as tag protein using E.coli or baculovirus or mammalian expression system of your choice. For this service all we need from you is the name of gene or accession number.
E.coli expression system is very useful to produce large amount of recombinant proteins comparatively at lower cost. We provide this service to generate fusion proteins in small to large quantities. A variety of tags (such as GST, 6xHis, MBP) are possible to add at N- or C-terminus. E.coli system’s limitations are lack of post-translation modifications and improper folding of expressed mammalian recombinant proteins. For mammalian proteins that require certain post-translation modification to retain biological activity, it is recommended to use baculovirus or mammalian expression system.
Baculovirus expression system has been very useful for the expression and purification of mammalian proteins. We offer this service for His tag or GST tag fusion protein production in micrograms to grams quantities. Full service is available for this expression system. You can also provide us recombinant baculovirus for stock amplification and recombinant protein expression in sf9 cells.
Those Investigators who prefer to use mammalian expression system for the production of biologically active recombinant proteins can take advantage of our full service. However, we also offer recombinant protein purification service if you provide us expression ready construct. We have capability to purify recombinant protein up to grams quantities using HEK cells.
Please contact us for In vitro Translation and Purification services.
Gene Synthesis & Cloning Services
Clonetex offers state of the art gene synthesis service with no set up fee. We try our best to achieve synthesis of difficult genes with sequence repeats or GC rich contents. We codon-optimize gene for expression in organism of your choice as well as sub-cloned it into pUC or pBSK vector with no extra charge. We can sub-clone your gene into vector of your choice for a nominal fee. You'll have to provide vector of your choice. For a quick non-binding quote please e-mail gene sequence to cs@clonetex.com .
Constructs for Adenovirus Expression System
It is a very strong and efficient system for the simultaneous expression of two or more specific gene(s) in mammalian cells. We provide this service for the construction of your gene of interest. Toxin-free final product is ready to transfect HEK cells for the production of recombinant adenovirus.
Constructs for Baculovirus Expression System
One of the most widely used gene(s) expression system for production of recombinant proteins in Eukaryotic cells (such as sf9, Sf21). We offer cloning services for the construction of gene of your interest in baculovirus-based expression vector(s). Final product is ready to transfect insect cells for the packaging of recombinant baculovirus.Construction of Gene-specific SiRNA
This revolutionary technique to block the expression of a specific protein(s) in cells or animals has proven to be a reliable and quick approach. We'll take care of your SiRNA construct from designing of the specific primers to its cloning in a SiRNA expression vector. We also provide SiRNA duplex synthesis service with desired modifications such as biotin.This service is provided for sub-cloning of gene/cDNA into vector of your choice. For accuracy, recombinant clones will be verified by restriction analysis. A project report with detail analysis and 10 mg of final product will be supplied. Customer supplies the specific gene/ cDNA and vector.
PCR
We'll design and make gene-specific primers for PCR amplification from a cDNA library. We'll buy commercially available specific cDNA library if customer can not provide it. PCR amplified cDNA fragment will be sub-cloned in TA cloning vector or in a vector of your choice. Recombinant clones will be verified by restriction fragment analysis. For accuracy, two selected clones will be sequenced. A project report with detail analysis and 10 mg of final product will be supplied.
RT-PCR
We'll reverse transcribe and PCR amplify specific gene from customer’s provided total RNA/mRNAs. Amplified fragment will be ligated in TA vector or in vector of your choice. Two clones will be sequenced for the validity of clones. A project report with detail analysis and 10 mg of final product will be supplied.
Antibody Production & Custom Peptide Synthesis
Custom Sera (70-Day Protocol) Packages
Package |
Custom Service |
List Price |
|---|---|---|
| CSI-PA1 | Std 70-Day Protocol including: ■ 1 Rabbits ■ 6 Immunizations/Rabbit ■ 1 Pre-immune Bleed ■ 2 Production Bleeds ■ 5 ELISA tests ■ Design and synthesis of 5 mg of <90% pure peptide ■ Conjugation of 1.5-2mg of peptide to carrier protein ■ 2.0 mg of Free peptide to customer | $750 |
| CSI-PA2 | Std 70-Day Protocol including: ■ 2 Rabbits ■ 6 Immunizations/Rabbit ■ 1 Pre-immune Bleed ■ 2 Test bleeds ■ 2 Production Bleeds ■ 5 ELISA tests (on all five bleeds) ■ Design and synthesis of 10 mg of <90% pure peptide ■ Conjugation of 3-4 mg of peptide to carrier protein ■ 2.5 mg of Free peptide to customer | $950 |
| CSI-PA3 | CSI-PA1 package plus Peptide-Affinity purification of production Bleed. | $1495 |
| CSI-PA4 | CSI-PA2 package but without any Design, synthesis, conjugation or Free peptide. Customer provides a Pre-conjugated, ready-to-inject antigen. | $800 |
Custom Anti-sera Production in Rabbits (Standard 70 Day-Protocol)
Days in protocol |
Procedure |
Sera/ Rabbit |
Service Performed |
|---|---|---|---|
| Day 0/ Wk-1 | Pre-immune bleed from rabbits & Immunization I | 2.5-5 ml | ELISA using peptide |
| Day 14/ Wk-2 | First boost SC | none | Physical check up of animals |
| Day 28/ Wk-4 | Second boost SC. First test bleed | 5 ml | ELISA using peptide. *Serum shipped to investigator |
| Day 42/ Wk-6 | Third boost SC. Second test bleed | 5 ml | ELISA using peptide. *Serum shipped to investigator |
| Day 56/ Wk-8 | Fourth boost SC. First production bleed | 15-20 ml | ELISA using peptide. *Serum shipped to investigator |
| Day 70/ Wk-10 | Fifth boost SC. Second production bleed | 15-20 ml | ELISA using peptide. *Serum shipped to investigator |
* All bleeds are shipped together unless requested.
Conjugation
The customer must select one carrier protein and one conjugation chemistry (MBS, EDC, Bdt or Glutaraldehyde) for the conjugation of the peptide/protein/antigen.
Amount of Peptide/Protein Antigen for Conjugation |
Carrier Protein |
|||
|---|---|---|---|---|
KLH |
BSA |
Ovalbumin |
Other |
|
| 3-4 mgs | $125 | $125 | $125 | Enquire |
| 5-8 mgs | $225 | $225 | $225 | Enquire |
Options after Completion of 70 Day-Standard Protocol.
Catalog # |
Options |
Service |
Charges/ Rabbit |
|---|---|---|---|
| PROJ-01 | Termination of host animals | Termination of host animals WITHOUT collection of Serum | $75 |
| PROJ-02 | Exsanguination of host animals | Termination of host animals AFTER collecting 25-50 ml serum/Rabbit | $225 |
| PROJ-03 | Extension of Protocol | Maintenance of animals/ Immunization & 2 Production bleeds | $345/month |
Additional Services
Catalog # |
Service |
Description |
List Price |
|---|---|---|---|
| ELISA-01 | ELISA | ELISA on test & Pre-immune bleeds at 5 serial dilutions | $75 |
| AFP-S40 | Affinity Purification | Couple Peptide to affinity matrix, purify 40 ml sera, ELISA on purified sample | $850 |
| AFG-S40 | Protein-G Purification | Purify 40 ml sera on a Protein-A column, ELISA on purified sample | $925 |
| AFA-S40 | Protein A-purification | Purify 40 ml sera on a Protein-G column, ELISA on purified sample | $1025 |
Custom Peptide Synthesis
Following is the price guide line ($/amino acid) for Custom Peptide synthesis for peptide up to 30 amino acids in length. Quality assurance data (HPLC and MS) is provided for each order. Prices are in US$ and does not include shipping & handling charges. Discounts are available for bulk quantities.
Amount |
Purity |
||||||||
|---|---|---|---|---|---|---|---|---|---|
CRUDE |
DESALTED |
70% |
75% |
80% |
85% |
90% |
95% |
98% |
|
| 1 mg | 3.9 | 5.2 | 6.9 | 9.0 | 10.5 | 11.4 | 12.5 | 14.1 | 20.1 |
| 5 mg | 4.8 | 6.0 | 8.2 | 10.3 | 11.8 | 13.5 | 15.8 | 16.3 | 23.7 |
| 10 mg | 5.8 | 7.5 | 9.5 | 11.9 | 13.9 | 15.7 | 18.4 | 19.1 | 27.8 |
| 15 mg | 6.2 | 7.9 | 10.1 | 12.7 | 15.2 | 18.2 | 21.2 | 21.2 | 31.0 |
| 20 mg | 6.5 | 8.2 | 11.1 | 15.0 | 17.1 | 20.1 | 23.9 | 24.2 | 35.4 |
| 25 mg | 6.8 | 8.5 | 12.0 | 16.6 | 18.8 | 21.4 | 25.1 | 26.1 | 38.2 |
| 30 mg | 7.0 | 9.0 | 13.7 | 17.8 | 19.9 | 22.4 | 28.0 | 28.6 | 40.9 |
| 40 mg | 8.0 | 9.8 | 14.3 | 18.2 | 20.7 | 23.7 | 29.4 | 30.2 | 43.5 |
| 50 mg | 8.4 | 10.3 | 15.7 | 20.8 | 23.2 | 25.9 | 31.3 | 32.1 | 46.3 |
| 60 mg | 9.9 | 11.4 | 19.3 | 24.3 | 26.6 | 28.7 | 35.1 | 36.5 | 52.2 |
| 80 mg | 10.2 | 11.7 | 21.5 | 28.5 | 31.1 | 34.2 | 41.2 | 42.3 | 61.4 |
| 1000 mg | 35.5 | 38.0 | 66.8 | 94.0 | 96.9 | 104.2 | 112.5 | 125.0 | 186.4 |
Amino acid modifications
Some of the modifications of peptides we offer are listed below. Please enquire about other possible modifications. The prices listed below are for modification of 10 mg of peptide.
Modification |
Price |
|---|---|
| Acetylation (N-Terminal) | Free |
| Amidation (C-Terminal) | Free |
| Biotin (N-Terminal) | $75 |
| Biotin (C-Terminal) | $215 |
| Aminohexanoic acid (Ahx) | $49 |
| Citrulline (Cit) | $49 |
| Hydroxyproline (Hyp) | $49 |
| Myristic acid (Myr) | $49 |
| Ornithine (Orn) | $49 |
| Phosphorylation-Serine | $170 |
| Phosphorylation-Threonine | $170 |
| Phosphorylation-Tyrosine | $170 |
| Cyclization | $215 |
| Disulfide Bridge | $215 |
Fluorescence/Dye Labeling |
Price |
|---|---|
| FITC | $170 |
| FAM | $170 |
| TAMRA | $215 |
Protein Carrier Conjugation |
Price |
|---|---|
| KLH (Keyhole Limpet hemocyanin) | $215 |
| BSA (Bovine serum albumin) | $215 |
| OVA (Ovalbumin) | $265 |
Multiple Antigenic Peptide (MAP) System |
Price |
|---|---|
| Asymmetric 4 branches | $150 |
| Symmetric 4 branches | $150 |
| Asymmetric 8 branches | $150 |
| Symmetric 8 branches | $150 |